3MPJ
Structure of the glutaryl-coenzyme A dehydrogenase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-11-15 |
| Detector | mar225 |
| Wavelength(s) | 1.000 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 152.400, 250.600, 62.800 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.000 - 2.100 |
| R-factor | 0.18317 |
| Rwork | 0.181 |
| R-free | 0.22466 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1siq |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.641 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.160 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.113 | 0.570 |
| Number of reflections | 133695 | |
| <I/σ(I)> | 11 | 3.1 |
| Completeness [%] | 99.7 | 98 |
| Redundancy | 6 | 5.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 277 | 1 mM FAD, 50% (v,v) MPD, 0.1 M Tris/HCl, pH 8.5 and 0.2 M NH4H2PO4, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
