3MNE

Crystal structure of the agonist form of mouse glucocorticoid receptor stabilized by F608S mutation at 1.96A

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X10SA
Synchrotron site	SLS
Beamline	X10SA
Temperature [K]	100
Collection date	2008-07-07
Wavelength(s)	0.7
Spacegroup name	P 65
Unit cell lengths	71.820, 71.820, 128.650
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	12.980 - 1.960
R-factor	0.1527
Rwork	0.151
R-free	0.18530
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1m2z
RMSD bond length	0.011
RMSD bond angle	1.053
Phasing software	PHASER
Refinement software	BUSTER-TNT (2.5.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	12.900	2.060
High resolution limit [Å]	1.960	1.960
Number of reflections	26835
<I/σ(I)>	15.72	2.41
Completeness [%]	99.7
Redundancy	11.22	9.18

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7		0.5 M ammonium sulfate, 0.9 M sodium tartrate, 0.1 M PIPES pH 7.0, VAPOR DIFFUSION, SITTING DROP