3MN8
Structure of Drosophila melanogaster carboxypeptidase D isoform 1B short
Experimental procedure
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID23-1 |
Synchrotron site | ESRF |
Beamline | ID23-1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-04-02 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.9724 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 97.020, 135.700, 141.710 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 49.000 - 2.700 |
R-factor | 0.2116 |
Rwork | 0.211 |
R-free | 0.28170 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1h8l |
RMSD bond length | 0.008 |
RMSD bond angle | 1.118 |
Data reduction software | XDS |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | PHENIX ((phenix.refine)) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 49.000 |
High resolution limit [Å] | 2.700 |
Number of reflections | 52035 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 277.15 | 8% PEG 4000, 0.2M KSCN, 0.1M sodium cacodylate, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 277.15K |