3MMY
Structural and functional analysis of the interaction between the nucleoporin Nup98 and the mRNA export factor Rae1
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 23-ID-B |
Synchrotron site | APS |
Beamline | 23-ID-B |
Detector technology | CCD |
Collection date | 2009-12-17 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 1.14014 |
Spacegroup name | P 1 |
Unit cell lengths | 56.396, 79.298, 93.407 |
Unit cell angles | 76.63, 89.96, 89.94 |
Refinement procedure
Resolution | 19.820 - 1.650 |
R-factor | 0.20769 |
Rwork | 0.206 |
R-free | 0.23742 |
Structure solution method | AB INITIO PHASING |
RMSD bond length | 0.010 |
RMSD bond angle | 1.260 |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 20.000 |
High resolution limit [Å] | 1.650 |
Number of reflections | 188056 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | VAPOR DIFFUSION, HANGING DROP |