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3MMY

Structural and functional analysis of the interaction between the nucleoporin Nup98 and the mRNA export factor Rae1

Experimental procedure
Experimental methodSINGLE WAVELENGTH
Source typeSYNCHROTRON
Source detailsAPS BEAMLINE 23-ID-B
Synchrotron siteAPS
Beamline23-ID-B
Detector technologyCCD
Collection date2009-12-17
DetectorADSC QUANTUM 315
Wavelength(s)1.14014
Spacegroup nameP 1
Unit cell lengths56.396, 79.298, 93.407
Unit cell angles76.63, 89.96, 89.94
Refinement procedure
Resolution19.820 - 1.650
R-factor0.20769
Rwork0.206
R-free0.23742
Structure solution methodAB INITIO PHASING
RMSD bond length0.010
RMSD bond angle1.260
Refinement softwareREFMAC (5.2.0019)
Data quality characteristics
 Overall
Low resolution limit [Å]20.000
High resolution limit [Å]1.650
Number of reflections188056
Crystallization Conditions
crystal IDmethodpHtemperaturedetails
1VAPOR DIFFUSION, HANGING DROPVAPOR DIFFUSION, HANGING DROP

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