3MMN

Crystal structure of the receiver domain of the histidine kinase CKI1 from Arabidopsis thaliana complexed with Mg2+

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	EMBL/DESY, HAMBURG BEAMLINE BW7B
Synchrotron site	EMBL/DESY, HAMBURG
Beamline	BW7B
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2009-01-05
Detector	MAR scanner 345 mm plate
Wavelength(s)	0.8123
Spacegroup name	C 2 2 21
Unit cell lengths	54.142, 100.915, 80.139
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	5.000 - 2.200
R-factor	0.17716
Rwork	0.174
R-free	0.23712
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3mm4
RMSD bond length	0.025
RMSD bond angle	1.905
Data reduction software	XDS
Data scaling software	XDS
Phasing software	MOLREP
Refinement software	REFMAC (5.5.0102)

Data quality characteristics

	Overall
Low resolution limit [Å]	5.000
High resolution limit [Å]	2.200
Number of reflections	10328

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.05	293	0.1M MES, Amm. sulphate, pH 5.05, vapor diffusion, hanging drop, temperature 293K