3MLP
Early B-cell Factor 1 (Ebf1) bound to DNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.2 |
| Synchrotron site | BESSY |
| Beamline | 14.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-08-14 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 0.9537 |
| Spacegroup name | P 1 |
| Unit cell lengths | 70.490, 78.690, 103.570 |
| Unit cell angles | 88.99, 90.00, 77.81 |
Refinement procedure
| Resolution | 38.750 - 2.800 |
| R-factor | 0.20661 |
| Rwork | 0.204 |
| R-free | 0.26411 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3mln |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.411 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.000 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Number of reflections | 52163 | |
| <I/σ(I)> | 9.9 | 2.3 |
| Completeness [%] | 96.7 | 96.2 |
| Redundancy | 2.3 | 2.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.4 | 310 | 10% PEG4000, 10% isopropanol, 100mM sodium citrate, pH 5.4, VAPOR DIFFUSION, HANGING DROP, temperature 310K |
