3MLN
DNA binding domain of Early B-cell Factor 1 (Ebf1) bound to DNA (crystal form II)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-08-03 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 71.200, 100.700, 72.000 |
| Unit cell angles | 90.00, 101.20, 90.00 |
Refinement procedure
| Resolution | 29.310 - 2.400 |
| R-factor | 0.17338 |
| Rwork | 0.171 |
| R-free | 0.21861 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3mlo |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.350 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.310 | 2.500 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Number of reflections | 38953 | |
| <I/σ(I)> | 14.8 | 3.2 |
| Completeness [%] | 99.6 | 98 |
| Redundancy | 4.6 | 4.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.4 | 293 | 10% PEG-4000, 200mM KCl, pH 7.4, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
