3MLG
2ouf-2x, a designed knotted protein
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-C |
| Synchrotron site | APS |
| Beamline | 24-ID-C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-02-20 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97910 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 44.942, 75.390, 118.573 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 21.990 - 2.290 |
| R-factor | 0.25 |
| Rwork | 0.248 |
| R-free | 0.27800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2OUF |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.150 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER (2.1.4) |
| Refinement software | BUSTER-TNT |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 90.000 | 90.000 | 2.380 |
| High resolution limit [Å] | 2.290 | 4.950 | 2.300 |
| Rmerge | 0.068 | 0.037 | 0.451 |
| Number of reflections | 17338 | ||
| <I/σ(I)> | 9.6 | ||
| Completeness [%] | 91.8 | 95.1 | 43.2 |
| Redundancy | 6.8 | 6.2 | 6.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.2 | 298 | 8% PEG 8000, 0.1 M Na+/K+ phosphate pH 6.2, 0.1 M sodium chloride, vapor diffusion, hanging drop, temperature 298K |
