3MLF
Putative transcriptional regulator from Staphylococcus aureus.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-02-10 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9792 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 159.776, 159.776, 69.344 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 44.300 - 2.600 |
| R-factor | 0.21 |
| Rwork | 0.208 |
| R-free | 0.24800 |
| Structure solution method | SAD |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.659 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SHELXD |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.300 | 2.640 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Rmerge | 0.146 | 0.790 |
| Number of reflections | 27459 | |
| <I/σ(I)> | 8.3 | 2.8 |
| Completeness [%] | 97.5 | 99.4 |
| Redundancy | 7 | 7.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 294 | 0.1 M TRIS buffer, 1.4 M di-ammonium tartrate, 1 mM magnesium chloride, pH 8.0, VAPOR DIFFUSION, SITTING DROP, temperature 294K |
