3ML4
Crystal structure of a complex between Dok7 PH-PTB and the MuSK juxtamembrane region
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X4C |
| Synchrotron site | NSLS |
| Beamline | X4C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-10-01 |
| Detector | ADSC QUANTUM 4r |
| Wavelength(s) | 0.97854 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 134.600, 134.600, 121.580 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 50.000 - 2.600 |
| R-factor | 0.25518 |
| Rwork | 0.253 |
| R-free | 0.30222 |
| Structure solution method | SAD |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.091 |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.690 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Rmerge | 0.098 | 0.357 |
| Number of reflections | 39533 | |
| <I/σ(I)> | 10 | 4.8 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 11.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 290 | 0.7 M Na-K tartrate, VAPOR DIFFUSION, HANGING DROP, temperature 290K |
