3MKZ
Structure of SopB(155-272)-18mer complex, P21 form
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.3.1 |
| Synchrotron site | ALS |
| Beamline | 8.3.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-10-12 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 111.380, 47.120, 118.230 |
| Unit cell angles | 90.00, 115.97, 90.00 |
Refinement procedure
| Resolution | 50.070 - 2.980 |
| R-factor | 0.213 |
| Rwork | 0.213 |
| R-free | 0.26400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3mkw |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.400 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | CNS (1.2) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 99.000 |
| High resolution limit [Å] | 2.980 |
| Rmerge | 0.067 |
| Number of reflections | 22277 |
| <I/σ(I)> | 7.3 |
| Completeness [%] | 99.0 |
| Redundancy | 2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 298 | PEG 400, Calcium chloride 200 mM, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
