3MKS
Crystal Structure of yeast Cdc4/Skp1 in complex with an allosteric inhibitor SCF-I2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 14-BM-C |
| Synchrotron site | APS |
| Beamline | 14-BM-C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-04-12 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.9 |
| Spacegroup name | P 32 |
| Unit cell lengths | 108.281, 108.281, 165.594 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 93.660 - 2.600 |
| R-factor | 0.21334 |
| Rwork | 0.211 |
| R-free | 0.26648 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1nex |
| RMSD bond length | 0.019 |
| RMSD bond angle | 1.968 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | CNS |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 93.660 | 2.688 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Rmerge | 0.058 | 0.435 |
| Number of reflections | 66846 | |
| <I/σ(I)> | 24.3 | 3.6 |
| Completeness [%] | 99.8 | 100 |
| Redundancy | 5.5 | 5.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 298 | 1.5 M Ammonium sulfate, 100mM Tris pH 8.5, 15% glycerol, 1mM SCF-I2, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
