3MKS
Crystal Structure of yeast Cdc4/Skp1 in complex with an allosteric inhibitor SCF-I2
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 14-BM-C |
Synchrotron site | APS |
Beamline | 14-BM-C |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2004-04-12 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 0.9 |
Spacegroup name | P 32 |
Unit cell lengths | 108.281, 108.281, 165.594 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 93.660 - 2.600 |
R-factor | 0.21334 |
Rwork | 0.211 |
R-free | 0.26648 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1nex |
RMSD bond length | 0.019 |
RMSD bond angle | 1.968 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | CNS |
Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 93.660 | 2.688 |
High resolution limit [Å] | 2.600 | 2.600 |
Rmerge | 0.058 | 0.435 |
Number of reflections | 66846 | |
<I/σ(I)> | 24.3 | 3.6 |
Completeness [%] | 99.8 | 100 |
Redundancy | 5.5 | 5.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 298 | 1.5 M Ammonium sulfate, 100mM Tris pH 8.5, 15% glycerol, 1mM SCF-I2, VAPOR DIFFUSION, HANGING DROP, temperature 298K |