3MK9
Stabilized Ricin Immunogen 1-33/44-198
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | BRUKER AXS MICROSTAR |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-02-21 |
| Detector | BRUKER SMART 6000 |
| Wavelength(s) | 1.54 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 51.744, 72.767, 96.382 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 58.030 - 2.080 |
| R-factor | 0.21108 |
| Rwork | 0.210 |
| R-free | 0.23603 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ifs |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.111 |
| Data reduction software | SAINT |
| Data scaling software | SADABS |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 58.070 | 2.170 |
| High resolution limit [Å] | 2.080 | 2.080 |
| Number of reflections | 10903 | |
| <I/σ(I)> | 20.18 | 3.88 |
| Completeness [%] | 96.6 | 77.1 |
| Redundancy | 9.61 | 3.01 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.4 | 290 | Protein Buffer: 50mM MES pH 6.4, 200mM NaCl, 2mM BME, Crystallization Solution: 0.17M Ammonium Sulfate, 25.5% w/v PEG 4000, 15% v/v Glycerol , VAPOR DIFFUSION, HANGING DROP, temperature 290K |
