3MJD
1.9 Angstrom Crystal Structure of Orotate Phosphoribosyltransferase (pyrE) Francisella tularensis.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-G |
| Synchrotron site | APS |
| Beamline | 21-ID-G |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-03-24 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 0.97856 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 82.553, 97.903, 110.724 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.470 - 1.900 |
| R-factor | 0.18802 |
| Rwork | 0.186 |
| R-free | 0.23477 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1oro |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.241 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.930 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.067 | 0.440 |
| Number of reflections | 71519 | |
| <I/σ(I)> | 23.7 | 4.4 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 6.4 | 6.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 295 | Protein solution: 7.5 mg/mL, 0.25M Sodium Chloride, 0.01M Tris-HCL (pH 8.3); Screen solution: Classics II G1, 0.2M Sodium chloride, 0.1M TRIS (pH 8.5), 25% PEG 3350., VAPOR DIFFUSION, SITTING DROP, temperature 295K |
