3MJ7

Crystal structure of the complex of JAML and Coxsackie and Adenovirus receptor, CAR

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-D
Synchrotron site	APS
Beamline	23-ID-D
Temperature [K]	100
Detector technology	CCD
Collection date	2007-12-01
Detector	MARMOSAIC 300 mm CCD
Spacegroup name	P 31 1 2
Unit cell lengths	89.865, 89.865, 127.170
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	38.920 - 2.800
R-factor	0.229
Rwork	0.227
R-free	0.27600
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	1.261
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	REFMAC (5.4.0069)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.000	2.900
High resolution limit [Å]	2.800	2.800
Rmerge	0.091	0.529
Number of reflections	14679
<I/σ(I)>	12.7	2.9
Completeness [%]	99.7	99.8
Redundancy	4.6	4.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6	295	0.25 M LI-SULFATE, 0.1 M MES, 24% PEG 3350, pH 6.00, VAPOR DIFFUSION, SITTING DROP, temperature 295K