3MJ6

Crystal structure of the gammadelta T cell costimulatory receptor Junctional Adhesion Molecule-Like Protein, JAML

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.2.1
Synchrotron site	ALS
Beamline	8.2.1
Temperature [K]	100
Detector technology	CCD
Collection date	2005-02-17
Detector	ADSC QUANTUM 210
Wavelength(s)	1.07812, 0.99984
Spacegroup name	P 43
Unit cell lengths	61.946, 61.946, 82.477
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	50.000 - 2.190
R-factor	0.172
Rwork	0.170
R-free	0.21300
Structure solution method	MAD
Starting model (for MR)	OBTAINED BY MAD PHASING OF A TA6BR12 SOAKED JAML CRYSTAL AND SUBSEQUENT MR INTO THE NATIVE HIGH RESOLUTION DATA
RMSD bond length	0.016
RMSD bond angle	1.716
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	REFMAC (5.2.0019)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.240
High resolution limit [Å]	2.190	2.190
Rmerge	0.083	0.415
Number of reflections	15924
<I/σ(I)>	11.1	2
Completeness [%]	99.7	99.2
Redundancy	3.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.25	295	3.25 M NA-FORMATE, 0.1 M IMIDAZOLE, pH 7.25, VAPOR DIFFUSION, SITTING DROP, temperature 295K