3MIU
Structure of Banana Lectin-pentamannose complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2005-08-08 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.54 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 80.911, 80.911, 147.405 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 24.920 - 2.630 |
| R-factor | 0.21875 |
| Rwork | 0.217 |
| R-free | 0.24335 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1x1v |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.279 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.720 |
| High resolution limit [Å] | 2.630 | 2.630 |
| Rmerge | 0.066 | 0.428 |
| Number of reflections | 17137 | |
| <I/σ(I)> | 33 | 4.9 |
| Completeness [%] | 99.7 | 98.7 |
| Redundancy | 8.76 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 298 | ZINC ACETATE DIHYDRATE, SODIUM CACODYLATE, 1,6-HEXANEDIOL, pH 8.0, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
