3MIS
I-MsoI re-designed for altered DNA cleavage specificity (-8G)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 93 |
| Detector technology | IMAGE PLATE |
| Collection date | 2008-06-01 |
| Detector | RIGAKU RAXIS IV++ |
| Wavelength(s) | 1.54 |
| Spacegroup name | P 1 |
| Unit cell lengths | 42.589, 42.632, 71.577 |
| Unit cell angles | 72.86, 72.83, 71.17 |
Refinement procedure
| Resolution | 23.247 - 2.300 |
| R-factor | 0.2488 |
| Rwork | 0.246 |
| R-free | 0.30780 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1m5x |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.138 |
| Data reduction software | CrystalClear |
| Data scaling software | CrystalClear |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((phenix.refine)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 23.250 | 2.380 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.043 | 0.307 |
| Number of reflections | 18927 | |
| <I/σ(I)> | 15 | 3.4 |
| Completeness [%] | 95.9 | 90.3 |
| Redundancy | 3.92 | 3.87 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.3 | 291 | 24% PEG 4000, 100mM Tris, 20mM NaCl, 5mM CaCl2 , pH 7.3, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
