3MIJ
Crystal structure of a telomeric RNA G-quadruplex complexed with an acridine-based ligand.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-02-01 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97630 |
| Spacegroup name | P 2 3 |
| Unit cell lengths | 56.606, 56.606, 56.606 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 16.340 - 2.600 |
| R-factor | 0.23603 |
| Rwork | 0.235 |
| R-free | 0.24752 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PDB ID 3IBK |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.018 |
| Data reduction software | CrystalClear |
| Data scaling software | CrystalClear |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0072) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 17.900 | 2.490 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.055 | 0.368 |
| Number of reflections | 2511 | |
| <I/σ(I)> | 16.1 | 4.3 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 6.6 | 6.78 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 285 | MPD, sodium chloride, potassium chloride, sodium cacodylate, potassium cacodylate, spermine, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 285K |
