3MII
Crystal structure of Y0R391Cp/HSP33 from Saccharomyces cerevisiae
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2005-07-01 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 96.420, 96.420, 132.230 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 25.500 - 2.400 |
| R-factor | 0.22337 |
| Rwork | 0.222 |
| R-free | 0.24570 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1qvz |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.946 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0072) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 60.630 | 2.530 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.118 | 0.443 |
| Total number of observations | 105739 | |
| Number of reflections | 45873 | |
| <I/σ(I)> | 8.7 | 2.3 |
| Completeness [%] | 97.3 | 99.4 |
| Redundancy | 2.3 | 2.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 5.6 | 298 | 18% PEG 3000, 200mM (NH4)2SO4, 100mM tri-Sodium Citrate dihytrate, pH 5.6, VAPOR DIFFUSION, temperature 298K |
