3MHD
Crystal structure of DCR3
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X29A |
| Synchrotron site | NSLS |
| Beamline | X29A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-08-15 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9791 |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 130.019, 130.019, 96.026 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 20.000 - 2.901 |
| R-factor | 0.28619 |
| Rwork | 0.285 |
| R-free | 0.31481 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3k51 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.212 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.3.0034) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.800 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Rmerge | 0.073 | 0.793 |
| Number of reflections | 8677 | |
| <I/σ(I)> | 18.9 | 1.2 |
| Redundancy | 7.3 | 5.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 290 | 0.2M sodium acetate, 1mM CaCl2, 0.1M sodium citrate, 10% PEG 4000, pH 5.5, VAPOR DIFFUSION, SITTING DROP, temperature 290K |
