3MH6
HtrA proteases are activated by a conserved mechanism that can be triggered by distinct molecular cues
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-12-12 |
| Detector | MARMOSAIC 225 mm CCD |
| Spacegroup name | F 4 3 2 |
| Unit cell lengths | 261.500, 261.500, 261.500 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 28.703 - 3.600 |
| R-factor | 0.275 |
| Rwork | 0.273 |
| R-free | 0.30910 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3cs0 |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.915 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.240 | 3.790 |
| High resolution limit [Å] | 3.600 | 3.600 |
| Rmerge | 0.243 | 0.787 |
| Number of reflections | 9340 | |
| <I/σ(I)> | 9.1 | 2.6 |
| Completeness [%] | 99.8 | 100 |
| Redundancy | 6.8 | 7.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 8.5 | 293 | 12% Isopropanol, 0.1M Tris, 12% PEG 2000 MME, pH 8.5, VAPOR DIFFUSION, temperature 293K |
