3MH4

HtrA proteases are activated by a conserved mechanism that can be triggered by distinct molecular cues

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-4
Synchrotron site	ESRF
Beamline	ID14-4
Temperature [K]	100
Detector technology	CCD
Collection date	2006-12-12
Detector	ADSC QUANTUM 315
Spacegroup name	P 63 2 2
Unit cell lengths	120.708, 120.708, 232.984
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	24.068 - 3.100
R-factor	0.2592
Rwork	0.257
R-free	0.30880
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1ky9
RMSD bond length	0.006
RMSD bond angle	0.936
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	CNS
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	25.000	3.210
High resolution limit [Å]	3.100	3.100
Rmerge	0.092	0.463
Number of reflections	18503
<I/σ(I)>	14.9	1.8
Completeness [%]	97.7	90
Redundancy	4.2	3.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	8.5	293	12% Isopropanol, 0.1M Tris, 12% PEG 2000 MME, pH 8.5, VAPOR DIFFUSION, temperature 293K