3MGI

Ternary complex of a DNA polymerase lambda loop mutant

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	277
Detector technology	IMAGE PLATE
Detector	RIGAKU
Wavelength(s)	1.504
Spacegroup name	P 21 21 21
Unit cell lengths	56.177, 63.235, 140.382
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	25.114 - 2.603
R-factor	0.203
Rwork	0.200
R-free	0.27000
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.003
RMSD bond angle	0.796
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	MOLREP
Refinement software	PHENIX

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	2.600
Number of reflections	15905
Completeness [%]	99.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	hanging drop	5.5	277	2-propanol, pH 5.5, hanging drop, temperature 277K