3MG6
Structure of yeast 20S open-gate proteasome with Compound 6
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 31-ID |
Synchrotron site | APS |
Beamline | 31-ID |
Detector technology | CCD |
Detector | MAR CCD 165 mm |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 137.253, 299.646, 145.785 |
Unit cell angles | 90.00, 113.67, 90.00 |
Refinement procedure
Resolution | 50.000 - 2.600 |
R-factor | 0.215 |
Rwork | 0.215 |
R-free | 0.24900 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1g0u |
RMSD bond length | 0.011 |
RMSD bond angle | 1.309 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 149.070 | 50.000 | 2.690 |
High resolution limit [Å] | 2.600 | 5.600 | 2.600 |
Rmerge | 0.092 | 0.050 | 0.353 |
Number of reflections | 315914 | ||
<I/σ(I)> | 11.1 | ||
Completeness [%] | 96.3 | 99.9 | 76.9 |
Redundancy | 7.1 | 7.6 | 3.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | 100 mM MES, 40 mM MgOAc, 15% 2-methyl-2,4-pentanediol (MPD), 10 mM EDTA, pH 7.0, vapor diffusion, hanging drop, temperature 293K |