3MG5
Core-streptavidin mutant F130L in complex with biotin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2008-08-30 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 50.910, 97.530, 52.680 |
| Unit cell angles | 90.00, 112.30, 90.00 |
Refinement procedure
| Resolution | 48.740 - 1.300 |
| R-factor | 0.134 |
| Rwork | 0.132 |
| R-free | 0.17800 |
| Structure solution method | isomorphous to another structure |
| Starting model (for MR) | 1mep |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.569 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | CCP4 |
| Refinement software | REFMAC (5.5.0047) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.350 |
| High resolution limit [Å] | 1.170 | 1.170 |
| Rmerge | 0.057 | 0.836 |
| Number of reflections | 121722 | |
| <I/σ(I)> | 14.1 | 0.7 |
| Completeness [%] | 76.0 | 14.5 |
| Redundancy | 4.6 | 2.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 0.1 M HEPES, pH 7.5, 16% PEG 8000, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
