3MG2
Crystal structure of the orange carotenoid protein Y44S mutant from cyanobacteria synechocystis sp. PCC 6803
Replaces: 3I1WExperimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.2.1 |
| Synchrotron site | ALS |
| Beamline | 8.2.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-05-15 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 32 |
| Unit cell lengths | 83.188, 83.188, 87.974 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 37.542 - 2.653 |
| R-factor | 0.1974 |
| Rwork | 0.194 |
| R-free | 0.25960 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3I1V |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.701 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.6_288) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 2.740 |
| High resolution limit [Å] | 2.650 | 5.710 | 2.650 |
| Rmerge | 0.129 | 0.037 | 0.687 |
| Number of reflections | 19757 | ||
| <I/σ(I)> | 7.1 | ||
| Completeness [%] | 100.0 | 99.9 | 100 |
| Redundancy | 5.6 | 5.8 | 5.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5 | 298 | 12% PEG 4000, 100mM Sodium acetate, 2% Glycerol, pH 5.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
