3MFM
Crystal Structures and Mutational Analyses of Acyl-CoA Carboxylase Subunit of Streptomyces coelicolor
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.2 |
| Synchrotron site | ALS |
| Beamline | 5.0.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-02-27 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | .99 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 79.668, 220.961, 136.742 |
| Unit cell angles | 90.00, 103.08, 90.00 |
Refinement procedure
| Resolution | 38.824 - 2.380 |
| R-factor | 0.2265 |
| Rwork | 0.224 |
| R-free | 0.26840 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.208 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | CNS |
| Refinement software | PHENIX (1.6.1_357) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 50.000 |
| High resolution limit [Å] | 2.380 |
| Rmerge | 0.100 |
| Number of reflections | 182058 |
| <I/σ(I)> | 3.75 |
| Completeness [%] | 99.0 |
| Redundancy | 6.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.8 | 298 | 0.1M Bis-TrispH 6.8, 10% MPD, 0.2M Ammonium acetate, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
