3MFE
Crystal Structure of Mycobacterium Tuberculosis Proteasome open-gate mutant with H0 movement
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X25 |
| Synchrotron site | NSLS |
| Beamline | X25 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-06-18 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.0809 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 118.968, 207.554, 142.275 |
| Unit cell angles | 90.00, 102.42, 90.00 |
Refinement procedure
| Resolution | 29.870 - 2.600 |
| R-factor | 0.224 |
| Rwork | 0.224 |
| R-free | 0.25700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3hfa |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.600 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.650 |
| High resolution limit [Å] | 2.560 | 2.560 |
| Rmerge | 0.076 | 0.327 |
| Number of reflections | 195691 | |
| <I/σ(I)> | 13.2 | 2.1 |
| Completeness [%] | 96.1 | 88.8 |
| Redundancy | 4.7 | 2.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.6 | 277 | 13.5% PEG 6000, 60mM sodium citrate, pH 5.8, 0.1M NaCl, VAPOR DIFFUSION, SITTING DROP, temperature 277.0K |
