3MF4
Crystal structure of putative two-component system response regulator/ggdef domain protein
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X29A |
| Synchrotron site | NSLS |
| Beamline | X29A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-04-29 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9791 |
| Spacegroup name | P 1 |
| Unit cell lengths | 40.919, 53.269, 57.908 |
| Unit cell angles | 61.95, 81.09, 78.76 |
Refinement procedure
| Resolution | 20.000 - 1.800 |
| R-factor | 0.224 |
| Rwork | 0.222 |
| R-free | 0.27300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3luf |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.285 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER (2.1.4) |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 1.830 |
| High resolution limit [Å] | 1.800 | 4.880 | 1.800 |
| Rmerge | 0.070 | 0.052 | 0.290 |
| Number of reflections | 33709 | ||
| <I/σ(I)> | 11.2 | ||
| Completeness [%] | 86.2 | 77.1 | 58.9 |
| Redundancy | 1.9 | 1.9 | 1.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 298 | 20% PEG 8000, 0.1 M Tris, 0.2 M MgCl2, pH 8.5, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
