3MEU

Crystal structure of SGF29 in complex with H3R2me2sK4me3

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-B
Synchrotron site	APS
Beamline	23-ID-B
Temperature [K]	100
Detector technology	CCD
Collection date	2009-11-18
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	1.04
Spacegroup name	P 21 21 21
Unit cell lengths	50.140, 65.483, 106.004
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	21.660 - 1.280
R-factor	0.20582
Rwork	0.205
R-free	0.22774
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3me9
RMSD bond length	0.011
RMSD bond angle	1.420
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Refinement software	REFMAC (5.5.0072)

Data quality characteristics

	Overall
Low resolution limit [Å]	55.710
High resolution limit [Å]	1.280
Number of reflections	88614

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.5	291	20-28% PEG3350, 0.1M Bis-Tris pH 5.5, vapor diffusion, Sitting drop, temperature 291K, VAPOR DIFFUSION, SITTING DROP