3MEM

Crystal structure of a Putative signal transduction protein (Maqu_0641) from MARINOBACTER AQUAEOLEI VT8 at 2.25 A resolution

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL9-2
Synchrotron site	SSRL
Beamline	BL9-2
Temperature [K]	100
Detector technology	CCD
Collection date	2010-01-24
Detector	MARMOSAIC 325 mm CCD
Wavelength(s)	0.91837,0.97911
Spacegroup name	P 21 21 2
Unit cell lengths	148.670, 53.070, 69.560
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	32.126 - 2.250
R-factor	0.187
Rwork	0.185
R-free	0.22700
Structure solution method	MAD
RMSD bond length	0.016
RMSD bond angle	1.563
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	SHELX
Refinement software	REFMAC (5.5.0109)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	32.126	32.126	2.310
High resolution limit [Å]	2.250	10.060	2.250
Rmerge	0.068	0.017	0.491
Number of reflections	26590	528	3372
<I/σ(I)>	9.39	38.1	1.5
Completeness [%]	97.9	93	89.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.2	277	0.2000M NaFormate, 20.0000% PEG-3350, No Buffer pH 7.2, NANODROP, VAPOR DIFFUSION, SITTING DROP, temperature 277K