3ME4
Crystal structure of mouse RANK
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM14 |
| Synchrotron site | ESRF |
| Beamline | BM14 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-09-03 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.9537 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 39.819, 94.245, 102.418 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.000 - 2.010 |
| R-factor | 0.20113 |
| Rwork | 0.199 |
| R-free | 0.23296 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3me2 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.066 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASES |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.070 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.096 | 0.644 |
| Number of reflections | 26472 | |
| <I/σ(I)> | 17.7 | 2.3 |
| Completeness [%] | 99.6 | 99.2 |
| Redundancy | 6.9 | 6.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 294 | 10% PEG 3350, 15% PEG 5000, 0.1M ammonium sulphate, 0.1M sodium tartrate, 0.05M MES pH6.5, VAPOR DIFFUSION, SITTING DROP, temperature 294K |
