3MDU

The structure of N-formimino-L-Glutamate Iminohydrolase from Pseudomonas aeruginosa complexed with N-Guanidino-L-Glutamate

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X4A
Synchrotron site	NSLS
Beamline	X4A
Temperature [K]	100
Detector technology	CCD
Collection date	2009-09-04
Detector	ADSC QUANTUM 4
Wavelength(s)	0.97915
Spacegroup name	I 41 2 2
Unit cell lengths	133.208, 133.208, 124.835
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	35.426 - 1.400
R-factor	0.18
Rwork	0.179
R-free	0.18910
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.006
RMSD bond angle	1.053
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	BALBES
Refinement software	PHENIX ((phenix.refine: 1.5_2))

Data quality characteristics

	Overall
Low resolution limit [Å]	35.426
High resolution limit [Å]	1.400
Rmerge	0.089
Number of reflections	102752
Completeness [%]	94.0

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	293	1.1M Sodium malonate, 0.1M Hepes, 0.5% Jeffamine, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 293.0K