3MDP

Crystal structure of a Putative Cyclic nucleotide-binding protein (Gmet_1532) from Geobacter metallireducens GS-15 at 1.90 A resolution

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL9-2
Synchrotron site	SSRL
Beamline	BL9-2
Temperature [K]	100
Detector technology	CCD
Collection date	2010-01-24
Detector	MARMOSAIC 325 mm CCD
Wavelength(s)	0.91162,0.97922,0.97898
Spacegroup name	P 4 21 2
Unit cell lengths	95.862, 95.862, 35.485
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	28.520 - 1.900
R-factor	0.183
Rwork	0.181
R-free	0.22700
Structure solution method	MAD
RMSD bond length	0.015
RMSD bond angle	1.511
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	SHELX
Refinement software	REFMAC (5.5.0102)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	28.520	28.520	1.970
High resolution limit [Å]	1.900	4.080	1.900
Rmerge	0.082	0.025	0.814
Number of reflections	13586	2527	2567
<I/σ(I)>	13.29	44.3	1.7
Completeness [%]	99.8	99.5	99.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	9	277	10.0000% PEG-6000, 0.1M Bicine pH 9.0, NANODROP, VAPOR DIFFUSION, SITTING DROP, temperature 277K