3MDF
Crystal structure of the RRM domain of Cyclophilin 33
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 4.2.2 |
| Synchrotron site | ALS |
| Beamline | 4.2.2 |
| Temperature [K] | 100 |
| Detector technology | NOIR1 |
| Collection date | 2008-04-22 |
| Detector | MBC DETECTOR |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 85.840, 40.520, 65.660 |
| Unit cell angles | 90.00, 127.06, 90.00 |
Refinement procedure
| Resolution | 26.200 - 1.850 |
| R-factor | 0.21 |
| Rwork | 0.210 |
| R-free | 0.23800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1cvj |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.340 |
| Data reduction software | d*TREK |
| Data scaling software | d*TREK |
| Phasing software | BALBES |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 26.200 | 1.920 |
| High resolution limit [Å] | 1.850 | 1.850 |
| Rmerge | 0.075 | 0.232 |
| Number of reflections | 15587 | |
| <I/σ(I)> | 15.6 | 5 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 6.76 | 6.41 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.6 | 291 | 0.1 M Hepes and 1.0 M tri-sodium dehydrate citrate pH 7.6, Microcapillary, temperature 291K |
