3MBZ

OXA-24 beta-lactamase complex soaked with 10mM SA4-17 inhibitor for 15min

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X29A
Synchrotron site	NSLS
Beamline	X29A
Temperature [K]	100
Detector technology	CCD
Collection date	2008-02-28
Detector	ADSC QUANTUM 315r
Wavelength(s)	1.29
Spacegroup name	P 41 21 2
Unit cell lengths	103.083, 103.083, 85.802
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	32.600 - 2.600
R-factor	0.17566
Rwork	0.174
R-free	0.21116
Structure solution method	FOURIER SYNTHESIS
RMSD bond length	0.011
RMSD bond angle	1.390
Refinement software	REFMAC (5.5.0088)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.690
High resolution limit [Å]	2.600	2.600
Rmerge	0.110	0.301
Number of reflections	13786
<I/σ(I)>	12.1	2.1
Completeness [%]	93.4	59.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.5		2M (NH3)2SO4, 0.1M Tris pH 8.5, room temperature, VAPOR DIFFUSION, SITTING DROP