3MA9
Crystal structure of gp41 derived protein complexed with fab 8066
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-04-03 |
| Detector | marccd 300 |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 41.527, 124.698, 133.657 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.450 - 2.050 |
| R-factor | 0.1943 |
| Rwork | 0.193 |
| R-free | 0.24512 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2cmr |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.209 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.120 |
| High resolution limit [Å] | 2.050 | 2.050 |
| Number of reflections | 44497 | |
| <I/σ(I)> | 15.24 | 1.92 |
| Completeness [%] | 99.2 | 96.6 |
| Redundancy | 4.8 | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 293 | 1.2M dibasic Ammonium phosphate, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
