3M9A
Protein structure of type III plasmid segregation TubR
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.3.1 |
| Synchrotron site | ALS |
| Beamline | 8.3.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-10-30 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.11587 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 32.494, 69.546, 114.217 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 57.110 - 2.500 |
| R-factor | 0.2234 |
| Rwork | 0.219 |
| R-free | 0.26395 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3m3e |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.267 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 59.400 | 2.640 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.066 | 0.384 |
| Number of reflections | 4637 | |
| <I/σ(I)> | 11.4 | 3 |
| Completeness [%] | 97.8 | 99.3 |
| Redundancy | 3.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.2 | 293 | 40 mM Na/K phosphate pH 6.2, 2.5 M NaCl, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
