3M99
Structure of the Ubp8-Sgf11-Sgf73-Sus1 SAGA DUB module
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.2.1 |
| Synchrotron site | ALS |
| Beamline | 8.2.1 |
| Temperature [K] | 77 |
| Detector technology | CCD |
| Collection date | 2009-05-06 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 64.424, 103.519, 70.350 |
| Unit cell angles | 90.00, 108.09, 90.00 |
Refinement procedure
| Resolution | 48.116 - 2.700 |
| R-factor | 0.237 |
| Rwork | 0.234 |
| R-free | 0.30000 |
| Structure solution method | SAD+MR |
| Starting model (for MR) | 2hd5 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.064 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHENIX |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | |
| High resolution limit [Å] | 2.700 | 2.700 |
| Number of reflections | 22908 | |
| <I/σ(I)> | 35.3 | 3.1 |
| Completeness [%] | 93.9 | 64.4 |
| Redundancy | 9.2 | 4.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.8 | 277 | 0.2M ammonium citrate, 18% PEG 3,350, pH 5.8, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
