3M7M
Crystal structure of monomeric hsp33
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PAL/PLS BEAMLINE 6B |
| Synchrotron site | PAL/PLS |
| Beamline | 6B |
| Temperature [K] | 93 |
| Detector technology | IMAGE PLATE |
| Collection date | 2002-05-11 |
| Detector | MAC Science DIP-2030 |
| Wavelength(s) | 1. |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 65.981, 65.981, 145.954 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.060 - 2.900 |
| R-factor | 0.22673 |
| Rwork | 0.224 |
| R-free | 0.28059 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1i7f |
| RMSD bond length | 0.018 |
| RMSD bond angle | 2.850 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 50.000 |
| High resolution limit [Å] | 2.900 |
| Rmerge | 0.066 |
| Number of reflections | 6752 |
| <I/σ(I)> | 11.8 |
| Completeness [%] | 91.5 |
| Redundancy | 4.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 298 | ammonium sulfate, lithium sulfate, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
