3M6Z
Crystal structure of an N-terminal 44 kDa fragment of topoisomerase V in the presence of guanidium hydrochloride
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 21-ID-G |
Synchrotron site | APS |
Beamline | 21-ID-G |
Temperature [K] | 78 |
Detector technology | CCD |
Collection date | 2008-07-24 |
Detector | RAYONIX MX-225 |
Wavelength(s) | 0.97872 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 63.640, 80.110, 137.220 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 28.900 - 1.400 |
R-factor | 0.16587 |
Rwork | 0.165 |
R-free | 0.18424 |
Structure solution method | SAD |
RMSD bond length | 0.009 |
RMSD bond angle | 1.195 |
Data reduction software | XDS |
Data scaling software | SCALA |
Phasing software | SHARP |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 28.900 | 1.460 |
High resolution limit [Å] | 1.400 | 1.400 |
Rmerge | 0.048 | 0.405 |
Number of reflections | 136662 | |
<I/σ(I)> | 27.5 | 5.3 |
Completeness [%] | 98.8 | 95.5 |
Redundancy | 8.5 | 7.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 303 | 0.1 M phosphate citrate pH 5.5, 0.15M sodium sulfate, 0.01M MgCl2, 1 M guanidium hydrochloride, 28% PEG 3350, VAPOR DIFFUSION, HANGING DROP, temperature 303K |
1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 303 | 0.1 M phosphate citrate pH 5.5, 0.15M sodium sulfate, 0.01M MgCl2, 1 M guanidium hydrochloride, 28% PEG 3350, VAPOR DIFFUSION, HANGING DROP, temperature 303K |