3M6Q
Crystal structure of Arabidopsis thaliana peptide deformylase 1B (AtPDF1B) G41Q mutant in complex with actinonin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-1 |
| Synchrotron site | ESRF |
| Beamline | ID14-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-02-23 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 0.934 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 56.630, 56.630, 146.550 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 45.000 - 2.400 |
| R-factor | 0.2327 |
| Rwork | 0.229 |
| R-free | 0.30850 |
| Structure solution method | rigid body |
| Starting model (for MR) | 3m6o |
| RMSD bond length | 0.029 |
| RMSD bond angle | 2.294 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.510 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Number of reflections | 10258 | |
| <I/σ(I)> | 22.77 | 6.1 |
| Completeness [%] | 98.8 | 93.5 |
| Redundancy | 12.9 | 11.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 20% PEG-3350, 0.1M zinc acetate, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
