3M6O
Crystal structure of Arabidopsis thaliana peptide deformylase 1B (AtPDF1B)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-12-16 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.980 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 55.970, 55.940, 148.550 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 27.970 - 2.000 |
| R-factor | 0.22052 |
| Rwork | 0.217 |
| R-free | 0.28377 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1rl4 |
| RMSD bond length | 0.022 |
| RMSD bond angle | 2.036 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.110 |
| High resolution limit [Å] | 2.000 | 1.990 |
| Number of reflections | 32457 | |
| <I/σ(I)> | 14.48 | 5.1 |
| Completeness [%] | 99.0 | 97.3 |
| Redundancy | 7.5 | 7.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 17% PEG-3350, 0.2M zinc acetate, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
