3M6K

Crystal Structure of N-terminal 44 kDa fragment of topoisomerase V in the presence of guanidium hydrochloride

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-G
Synchrotron site	APS
Beamline	21-ID-G
Temperature [K]	78
Detector technology	CCD
Collection date	2008-04-23
Detector	RAYONIX MX-225
Wavelength(s)	0.97872
Spacegroup name	P 41 21 2
Unit cell lengths	70.090, 70.090, 349.630
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.140 - 2.600
R-factor	0.24371
Rwork	0.241
R-free	0.28883
Structure solution method	SAD
RMSD bond length	0.011
RMSD bond angle	1.203
Data reduction software	XDS
Data scaling software	SCALA
Phasing software	SHARP
Refinement software	REFMAC (5.2.0019)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	29.500	2.720
High resolution limit [Å]	2.600	2.600
Rmerge	0.079	0.572
Number of reflections	28151
<I/σ(I)>	19	3.2
Completeness [%]	99.9	100
Redundancy	8.1	6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5	303	0.1 M phosphate-citrate pH 5, 0.2 M NaCl, 16% PEG 8000, 1M Guanidium hydrochloride, VAPOR DIFFUSION, HANGING DROP, temperature 303K
1	VAPOR DIFFUSION, HANGING DROP	5	303	0.1 M phosphate-citrate pH 5, 0.2 M NaCl, 16% PEG 8000, 1M Guanidium hydrochloride, VAPOR DIFFUSION, HANGING DROP, temperature 303K