3M5B
Crystal structure of the BTB domain from FAZF/ZBTB32
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE F2 |
| Synchrotron site | CHESS |
| Beamline | F2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-09-08 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 0.9790 |
| Spacegroup name | P 1 2 1 |
| Unit cell lengths | 49.076, 32.809, 69.425 |
| Unit cell angles | 90.00, 108.20, 90.00 |
Refinement procedure
| Resolution | 26.830 - 2.000 |
| R-factor | 0.18916 |
| Rwork | 0.186 |
| R-free | 0.24388 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | STARTING MODEL: SINGLE CHAIN POLY-ALANINE MODEL OF PDB CODE 1BUO EXCEPT LOOP REGIONS AND TURNS |
| RMSD bond length | 0.023 |
| RMSD bond angle | 1.792 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.050 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Number of reflections | 14434 | |
| <I/σ(I)> | 16.95 | 3.77 |
| Completeness [%] | 99.2 | 96.8 |
| Redundancy | 2.96 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 295 | 0.2 M ammonium sulfate, 0.35 M sodium citrate pH 5.5 and 30% (w/v) PEG 3350, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
