3M57
SET7/9 Y245A in complex with TAF10 peptide and AdoHcy
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-D |
| Synchrotron site | APS |
| Beamline | 23-ID-D |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-04-13 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 1.0093 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 83.636, 83.636, 96.053 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 33.890 - 1.700 |
| R-factor | 0.197 |
| Rwork | 0.196 |
| R-free | 0.22100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2f69 |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.613 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 35.000 | 35.000 | 1.760 |
| High resolution limit [Å] | 1.700 | 3.660 | 1.700 |
| Rmerge | 0.055 | 0.037 | 0.421 |
| Number of reflections | 42981 | ||
| <I/σ(I)> | 20.5 | ||
| Completeness [%] | 99.1 | 95.2 | 96.2 |
| Redundancy | 7.4 | 6.9 | 5.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 293 | 0.9 M Sodium Citrate, 0.1 M Imidazole pH 8, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
