3M4O
RNA polymerase II elongation complex B
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL11-1 |
| Synchrotron site | SSRL |
| Beamline | BL11-1 |
| Temperature [K] | 77 |
| Detector technology | CCD |
| Collection date | 2008-06-09 |
| Detector | MARMOSAIC 325 mm CCD |
| Wavelength(s) | 0.979 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 168.114, 222.131, 191.782 |
| Unit cell angles | 90.00, 100.99, 90.00 |
Refinement procedure
| Resolution | 40.000 - 3.570 |
| R-factor | 0.24391 |
| Rwork | 0.241 |
| R-free | 0.29233 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1r9t |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.568 |
| Data reduction software | DENZO |
| Data scaling software | HKL-2000 |
| Phasing software | MLPHARE |
| Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 3.660 |
| High resolution limit [Å] | 3.570 | 3.570 |
| Number of reflections | 70340 | |
| <I/σ(I)> | 6.3 | 1.4 |
| Completeness [%] | 92.3 | 92.7 |
| Redundancy | 2.5 | 2.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 298 | 390mM (NH4)2HPO4/NaH2PO4, 50mM dioxane, 10mM DTT, 9-11% PEG6,000, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | (NH4)2HPO4/NaH2PO4 | ||
| 2 | 1 | 1 | dioxane | ||
| 3 | 1 | 1 | DTT | ||
| 4 | 1 | 1 | PEG6,000 | ||
| 5 | 1 | 2 | (NH4)2HPO4/NaH2PO4 | ||
| 6 | 1 | 2 | dioxane | ||
| 7 | 1 | 2 | DTT | ||
| 8 | 1 | 2 | PEG6,000 |
