3M3U
Effect of temperature on tryptophan fluorescence in lysozyme crystals
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 293 |
| Detector technology | IMAGE PLATE |
| Collection date | 2005-07-16 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.514 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 79.095, 79.095, 38.108 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.770 - 2.350 |
| R-factor | 0.16742 |
| Rwork | 0.166 |
| R-free | 0.19924 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1bvx |
| RMSD bond length | 0.019 |
| RMSD bond angle | 1.753 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.5.0072) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.770 | 2.409 |
| High resolution limit [Å] | 2.350 | 2.349 |
| Rmerge | 0.065 | 0.163 |
| Number of reflections | 5384 | |
| <I/σ(I)> | 11.26 | 5.211 |
| Completeness [%] | 99.9 | 99.21 |
| Redundancy | 12.86 | 10.43 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.8 | 295 | 0.1M Sodium Acetate, 7% NaCl, 25% Ethylene glycol, pH 4.8, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
