3M3N
Structure of a Longitudinal Actin Dimer Assembled by Tandem W Domains
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 17-BM |
| Synchrotron site | APS |
| Beamline | 17-BM |
| Temperature [K] | 112 |
| Detector technology | CCD |
| Collection date | 2009-07-23 |
| Detector | MAR CCD 165 mm |
| Spacegroup name | P 65 2 2 |
| Unit cell lengths | 100.743, 100.743, 458.839 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 50.000 - 7.000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3m1f |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | AMoRE |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 7.250 |
| High resolution limit [Å] | 7.000 | 7.000 |
| Rmerge | 0.086 | 0.389 |
| Number of reflections | 2182 | |
| <I/σ(I)> | 16.5 | 1 |
| Completeness [%] | 90.0 | 34.3 |
| Redundancy | 20.5 | 2.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 10 | 300 | 100 mM CAPS pH 10.0, and 24% PEG 3350, 100 mM RbCl, VAPOR DIFFUSION, HANGING DROP, temperature 300.0K |
